t7 rna polymerase中文是什么意思

• t7rna聚合酶

• "rna"中文翻译    RNA ＝ribonucleic acid 【生物化学】 ...
• "polymerase"中文翻译    n. 【生物化学】聚合酶。
• "rna polymerase" 中文翻译 :    rna聚合酶; 核糖核酸聚合酶
• "t7 dna polymerase sequencing kit" 中文翻译 :    t7dna多聚酶测序试剂盒
• "rna dependent rna polymerase" 中文翻译 :    rna依赖性rna聚合酶
• "rna directed rna polymerase" 中文翻译 :    rna指导的rna聚合酶
• "rna-dependent rna polymerase" 中文翻译 :    依赖于rna的rna聚合酶
• "rna-directed rna polymerase" 中文翻译 :    rna-引导的rna聚合酶
• "rna-directed rna polymerase rna" 中文翻译 :    指导的rna聚合酶
• "rna-directed dna polymerase rna" 中文翻译 :    指导的dna聚合酶
• "core rna polymerase" 中文翻译 :    rna多聚核心酶
• "dna dependent rna polymerase" 中文翻译 :    依赖dna的rna聚合酶
• "dna directed rna polymerase" 中文翻译 :    dna指导性rna聚合酶
• "dna-directed rna polymerase" 中文翻译 :    dna指导的rna聚合酶
• "dnadependent rna polymerase" 中文翻译 :    依赖脱氧核糖核酸的核糖核酸聚
• "fmdv specific rna polymerase" 中文翻译 :    口蹄疫病毒特异性核糟核酸聚合酶
• "nucleolar rna polymerase" 中文翻译 :    核仁rna聚合酶
• "rn a-dependent rna polymerase" 中文翻译 :    依赖rna的rna聚合酶
• "rna dependent dna polymerase" 中文翻译 :    rna依赖性dna聚合酶
• "rna directed dna polymerase" 中文翻译 :    核糖核酸指导的脱氧核糖核酸聚合酶,rna指导的dna聚合酶
• "rna polymerase associated protein" 中文翻译 :    rna聚合酶相关蛋白
• "rna polymerase reaction in bacteria" 中文翻译 :    细菌中的
• "rna polymerase structure bacterialrna" 中文翻译 :    聚合酶结构
• "rna-dependent dna polymerase" 中文翻译 :    rna依赖性dna聚合酶,依赖rna的dna聚合酶,核糖核酸依赖性脱氧核糖核酸聚合酶; 核糖核酸依赖性脱氧核糖核酸聚合酶
• "rna-directed dna polymerase" 中文翻译 :    rna指导的dna聚合酶,核糖核酸指导的脱氧核糖核酸聚合酶; 核糖核酸指导的脱氧核糖核酸聚合酶

• Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase
  以线性化的全长cdna为模板，体外转录得到了csfv基因组rna 。
• To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
  方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。
• The fragments were subcloned into a low copy transcription vector ( px8dt ) between the t7 rna polymerase promoter and autocatalytic hepatitis delta virus ribozyme . the result showed that genome of ndv f48e9 strain comprises 15192 nt , which was equal to zjl strain and 6 nucleosides longer than that of la sota , v4 , bl and clone30
  实验结果表明： f48e9全基因组具有15192个碱基，比lasota 、 v4 、 b1和clone30的全基因组序列长6个碱基，和鹅源zj1株的长度相等。
• Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
  结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。
• Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed
  摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。
• 1 . expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes , an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro . the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
  将cry基因的高保守区的cry a ( a ) ecog - f片段插入带有t7rna聚合酶启动子的质粒pselect - 1 ，获得了能在体外转录的rna探针载体pbpl - 1 ，用该载体制备的rna探针具有特异强，背景清楚，省时省力等优点，已成功地用于苏云金芽胞杆菌的分子生物学研究和特异菌株的筛选。
• To prove that the cloned dna fragment can express tryptopanase , a new plasmid pet28c - tnaa , in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ) , a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter , which is inducible by iptg
  用iptg诱导表达t7rna聚合酶，以表达质粒上的目的基因。在葡萄糖存在的条件下，用常规方法发酵和诱导( 37 1mmiptg ) ，发现表达的蛋白质条带的分子量与理论上计算的分子量一致。但是发酵液中检测不到吲哚，表明虽然表达了目标蛋白，但表达的蛋白质没有酶活性。